Journal: Cell Reports Medicine

Article Title: Outer radial glia promotes white matter regeneration after neonatal brain injury

doi: 10.1016/j.xcrm.2025.101986

Figure Lengend Snippet:

Article Snippet: Alexa Fluor 488 AffiniPure Fab Fragment Donkey Anti-mouse IgG (H + L) , Jackson ImmunoResearch , Cat# 715-547-003; RRID: AB_2340851.

Techniques: Virus, Plasmid Preparation, Recombinant, RNAscope, Blocking Assay, Staining, Knock-Out, In Situ, Multiplex Assay, Sequencing, Software

Journal: bioRxiv

Article Title: eccDNA purification, full-length sequencing, and genomic mapping

doi: 10.1101/2022.06.15.496131

Figure Lengend Snippet: a) The 3SEP scheme. Step 1, crude DNA circles are extracted from whole cells with a buffered alkaline lysis at pH 11.8, bound and eluted from silica column; Step 2, linear DNA and PacI linearized mitochondrial DNA are digested with Plasmid-Safe (P.S.) DNase; Step 3, eccDNAs are selectively recovered with magnetic beads and solution A. b) EccDNA sequencing procedure. EccDNAs are firstly amplified by rolling cycle amplification and debranched with T7 endonuclease I (T7I), then read through by Oxford Nanopore. The full-length sequence of the eccDNA is called as consensus sequence of multiple tandem copies within long read.

Article Snippet: The output of an Oxford Nanopore sequencing run is the raw signals stored in multiple fast5 files. guppy will be used to perform base calling, which would convert the raw signals to DNA sequences stored in fastq format.

Techniques: Alkaline Lysis, Plasmid Preparation, Magnetic Beads, Sequencing, Amplification